Delivering four populations simultaneously to a purity frequently in excess of 99p percent, the FACSArias have been surpassing expectations. The Aria-I is configured with BD Biosciences’ patented octagon/trigon optical bench and new-age flow cell, providing fixed alignment with stream in air sorting. The FACSAria-I (BD) instrument is equipped with three lasers, capable of detecting up to 13 colors, and is a high-speed cell sorter. CellQuest Pro (v5.x) is the software driving this instrument. The four-color FACSCalibur (BD) analyzer is a two-laser instrument, capable of collecting a maximum of eight parameters: two scatter, four colors (green, yellow-orange, red and a second red) plus two derived parameters. The three-color FACScan (BD) analyzer is a one-laser instrument, capable of collecting a maximum of seven parameters: two scatter, three colors (green, yellow-orange and red) plus two derived parameters. We use BD Biosciences and Amnis instrumentation. The second floor FACS Core houses five cytometers, two analyzers and two sorters and a novel imaging flow cytometer (Imagestream).
